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Vastaanotettu Hilmaan	2023-03-27
Ilmoituksen numero	2023-124252
TED numero	2023/S 065-195108
Ostajaorganisaatio	Finnish Institute for Health and Welfare (2229500-6 ) P.O. Box 30 FI-00271 Helsinki https://thl.fi/en/web/thlfi-en
Hankinnan otsikkotiedot	EU Prior Information Notice (PIN) - Amplicon Sequencing Service for microbial community characterisation from environmental and human samples
Hankinnan yhteenlaskettu kokonaisarvo koko ajalle (ilman alv:ta) arvio
Hankinnan yhteenlaskettu kokonaisarvo koko ajalle (ilman alv:ta) lopullinen
Alkuperäinen ilmoitus	https://www.hankintailmoitukset.fi/fi/public/procurement/85295/notice/124252/overview
Originaali JSON tietue	124252.json

Hankinnan lyhyt kuvaus	Please note that this is a Prior Information Notice (PIN) only and not a Call for Tenders or an Invitation to Tender. * THL has collected and will collect additional samples from natural and man-made environments (indoor air, dust, water, etc.) and humans (skin swabs, nasal swabs, throat swabs, etc.) and extract total genomic DNA from those samples. In addition, from part of the samples, also total RNA has been or will be extracted. The objective of the procurement is to generate amplicon sequencing data from the collected samples. The microbiome targets include primarily bacteria (16S rRNA), microeukaryotes (18S rRNA) and fungi (ITS sequence) but may also include other targets eg. archaea or functional genes. Amplicon sequencing service (complete service from nucleic acids): THL will ship the nucleic acids (DNA and/or cDNA samples) to be sequenced, typically in 15-50 μl volume, to the service provider. THL will ship the samples in several batches. A typical batch size will be 100 to 500 samples, but batches may be also smaller or larger on occasion. THL expect the service provider to store the remainder of the sample batch for possible later sequencing up to one year and/or communicate with THL returning unused nucleic acids. THL also expect the service provider to discuss in detail PCR and sequencing conditions for each batch to be processed beforehand. Post PCR and prior to sequencing, THL request agarose gel pictures (or comparable technique) with sample identification that are used to decide on success of PCR amplification of the sample DNA. The exact total number of samples or the total number of batches per each amplicon target (bacteria, fungi, others) is not known. All batches will contain at least one negative and one positive control (mock community) sample. In total, we are anticipating to request bacterial, microeukaryote and fungal amplicon sequencing service of several batches between 100 and 500 samples within the first 12 months of the service contract. * The information provided in this notice is preliminary and the subject of the procurement may change.
Hankintanimikkeistö (CPV) pää	Research laboratory services (73111000)
Hankintanimikkeistö (CPV) muut
Aluekoodi	FI
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