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Vastaanotettu Hilmaan	2020-07-21
Ilmoituksen numero	2020-051228
TED numero	2020/S 141-347065
Ostajaorganisaatio	Finnish Institute for Health and Welfare (2229500-6 ) PL 30 FI-00271 Helsinki http://www.thl.fi/
Hankinnan otsikkotiedot	Next-generation Sequencing Services 2020-2022 (2023, 2024)
Hankinnan yhteenlaskettu kokonaisarvo koko ajalle (ilman alv:ta) arvio	200 000 EUR
Hankinnan yhteenlaskettu kokonaisarvo koko ajalle (ilman alv:ta) lopullinen
Alkuperäinen ilmoitus	https://www.hankintailmoitukset.fi/en/public/procurement/39135/notice/51228/overview
Originaali JSON tietue	51228.json

Hankinnan lyhyt kuvaus	THL has collected and will collect more samples from natural and man-made environments and humans and extract total genomic DNA from those samples. In addition, from part of the samples, also total RNA, viral DNA and viral RNA have been or will be extracted. The objective of the procurement is to gain microbiome sequencing data from the collected samples. The microbiome targets include primarily bacteria (16S rRNA) and fungi (ITS sequence), but may also include eg. archaea, viruses or protozoa. Amplicon sequencing service (complete service from nucleic acids): THL will ship the nucleic acids (DNA and/or cDNA samples) to be sequenced, typically in 15-50 µl volume, to the service provider. We will ship the samples in several batches. A typical batch size will be 100 to 500 samples, but batches may be also smaller or larger on occasion. We expect the service provider to store the remainder of the sample batch for possible later sequencing up to one year and/or communicate with THL returning unused nucleic acids. We also expect the service provider to discuss in detail PCR and sequencing conditions for each batch to be processed beforehand. Post PCR and prior to sequencing, we request agarose gel pictures (or comparable technique) with sample identification that are used to decide on success of PCR amplification of the sample DNA. The exact total number of samples or the total number of batches per each amplicon target (bacteria, fungi, others) is not known. All batches will contain at least one negative and one positive control (mock community) sample. In total, we are anticipating to request bacterial and fungal amplicon sequencing service of several batches between 100 and 500 samples within the first 12 months, and similar throughput for following years. The subject of procurement and the mandatory requirements relating to it are described in detail in this Call for Tender and its annexes.
Hankintanimikkeistö (CPV) pää	Research laboratory services (73111000)
Hankintanimikkeistö (CPV) muut	Research and development services and related consultancy services (73000000)
Aluekoodi	FI