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Hankkija
| Vastaanotettu Hilmaan | 2023-09-11 |
| Ilmoituksen numero | 2023-136340 |
| TED numero | 2023/S 178-554564 |
| Ostajaorganisaatio | University of Turku (0245896-3 ) Yliopistonmäki FI-20014 Turun yliopisto http://www.utu.fi |
| Hankinnan otsikkotiedot | Laser-scanning confocal microscope with time-correlated single photon counting (TCSPC) based fluorescence lifetime imaging microscopy (FLIM) |
| Hankinnan yhteenlaskettu kokonaisarvo koko ajalle (ilman alv:ta) arvio | |
| Hankinnan yhteenlaskettu kokonaisarvo koko ajalle (ilman alv:ta) lopullinen | 814 900 EUR |
| Alkuperäinen ilmoitus | https://www.hankintailmoitukset.fi/en/public/procurement/90617/notice/136340/overview |
| Originaali JSON tietue | 136340.json |
Ostettava
| Hankinnan lyhyt kuvaus | The University of Turku and Åbo Akademi University (for Turku Bioscience) intend to jointly purchase a state-of-the-art laser-scanning confocal microscope that combines conventional confocal intensity imaging, with an extended imaging range from far-UV (>400 nm) to near Infrared (<850 nm), and a fully integrated solution for time-correlated single photon counting (TCSPC) based fluorescence lifetime imaging microscopy (FLIM). This microscope is to be placed in the Cell Imaging and Cytometry (CIC) multi-user core facility. Thus, it is important that the purchased microscope can be configured to be broadly applicable to a large number of samples and imaging modalities, while a requirement for proven performance at technical specifications in similar research environments, ease of use, and ease of maintenance service and repairs is absolutely essential. The setup will be optimized for a broad range of sample types and imaging modalities, according to the research strongholds in Turku: I. Conventional confocal intensity imaging of live and fixed cells, tissue, zebrafish embryos, organoids, and spheroids II. Fluorescence lifetime imaging of a variety of biosensors and FLIM probes in live cells, tissue, spheroids, and zebrafish embryosIII. Conventional confocal imaging of thicker cleared tissue samplesFor required flexibility and ease of use in a multi-user core facility, the system to be purchased must include a capability of conventional confocal imaging with an extended excitation range from 405 nm to at least 790 nm, and an associated extended emission detection range from about 400 nm to 850 nm. This system should furthermore enable TCSPC based FLIM imaging of an extended imaging excitation range from 440 nm to 790 nm, and an associated extended emission detection range from about 450 nm to 850 nm. To enable these capabilities, this system should include at least five dedicated high-sensitivity point detectors to enable simultaneous imaging of 5 partially spectrally overlapping fluorophores. These detectors should further be capable of both conventional integrated counting and photon counting measurements, have freely tuneable AOBS based detection ranges, and should include one or more optimized near-IR detectors. Furthermore, this system must incorporate a CW 405 nm laser in combination with a pulsed white light laser with a tuneable excitation range from around 440 nm to at least 790 nm. In addition, for required flexibility and ease of use in a multi-user core facility, this system must include a FLIM data analysis solution that integrates both standard FLIM analysis fitting approaches to TCSPC histograms, and fit-free FLIM Phasor Analysis approaches. This system must also must include an integrated solution which enables improved imaging capabilities by providing a validated solution for enhanced contrast imaging by fluorescence lifetime-based separation of autofluorescence from specific fluorescence signal, and for enhanced multiplexing imaging capabilities much beyond the simultaneous imaging of 5 partially spectrally overlapping fluorophores by also enabling the differentiation of spectrally overlapping fluorophores from their differences in fluorescence lifetimes. Finally, for ease of use, in a multi-user core facility, and for ease of service maintenance, it is required that the purchased system is fully integrated in a single software application package, both in terms of data acquisition and data analysis, and that the system can be supplied and fully supported by a single service provider. |
| Hankintanimikkeistö (CPV) pää | Microscopes (38510000) |
| Hankintanimikkeistö (CPV) muut | Laboratory, optical and precision equipments (excl. glasses) (38000000) |
| Aluekoodi | FI1C1 |
| Pääasiallinen suorituspaikka |
Sopimukset
| Päätös päivämäärä | 2023-09-06 |
| Sopimusnumero | |
| Myyjät | Immuno Diagnostic Oy (FI), 0499964-0 |
