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                "The University of Turku and \u00c5bo Akademi University proposes to buy together a ThermoFisher ZipChip separation capillary electrophoresis (CZE) separation unit together with a Field Asymmetric Ion Mobility Separation unit (FAIMS). ",
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                "These units will be mounted (separately) to an existing ThermoFisher Lumos Orbitrap mass spectrometer. The Lumos is constructed in such a manner as to allow the analysis of intact proteins and to sequence them. Proteins cannot be reproducibly separated using the standard procedure of reverse-phase HPLC and so we need a CZE based separation. This allows separation of proteins that are modified (such as glycosylation, phosphorylation, deamidation etc.) which is essential for quality control of therapeutic proteins. The Universities also provide this type of service externally and the feature is therefore essential. In addition, FAIMS will allow to gain an extra dimension in peptide separation and by measuring the ion mobility increase the confidence levels of identifications. In addition, FAIMS opens up a novel approach to determining which drugs bind which protein.  The two add-on separation techniques are controlled by software unique to the ThermoFisher Lumos Orbitrap mass spectrometer and will greatly expand the Universities repertoire of analytical techniques.",
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                "The ZipChip unit is a miniaturised capillary zone electrophoresis separation device constructed as a microchip in a special holder that interfaces only to the ThermoFisher electrospray source. Commercial CZE instrumentation has been available since the 1990\u2019s. Now many companies offer CZE also in a chip format (such as PerkinElmer, Sciex, Agilent, Capilix, Isogen etc.). However, none of these other instruments can be controlled by the ThermoFisher software. This is essential since the main use is the ability to define the speed of separation to allow \u201cpeak parking\u201d. The peptides and proteins are separated by applying a voltage across the capillary and it is possible to slow down and almost completely stop the passage of analytes by lowering the voltage allowing the mass spectrometer almost indefinite time to carry out MS\/MS and MSn, analyses so greatly increasing coverage and depth of analysis. This requires online control of the separation that allows \u201cintelligent control\u201d of the separation. The alternative of using offline CZE separation is not feasible due to the extremely low analyte amounts and the fact that the throughput would be slowed down by around 50-fold. The ThermoFisher ZipChip is the only instrument format that can allow direct \u201cintelligent control\u201d of analyte separation since it requires direct control by the Orbitrap Instrument control language software.",
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