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"For in-depth proteome analyses from minute sample amount we needed QTOF type MS with ion mobility separation. Ion mobility is a great extension to MS that delivers information about the three-dimensional structure of an ion, and increases peak capacity and confidence in compound characterization. Other needed features were; ",
"1) The instrument can synchronize the quadrupole with the IMS elution time to allow for a high MS\/MS acquisition speed, leading to an increased fragmentation depth and there must be algorithms for intelligent targeting of low-level precursors",
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"4) Reproducibility of Collisional Cross Section (CCS) value determination <0.5 % RSD",
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"The timsTOF Pro system comes with a dual-TIMS (trapped ion mobility spectrometry) analyzer optimized for high-speed shotgun proteomics. It has nearly 100% duty cycle, making this parallel accumulation serial fragmentation technique (PASEF) perfectly suited for nanoflow LC-MS analysis of enzymatically digested complex protein mixtures. The timsTOF Pro from Bruker is the only instrument in the market that has TIMS technology allowing PASEF, dia-PASEF and prm-PASEF acquisition methods. We also have large-scale clinical applications where we plan to use this instrument in conjunction with Evosep One LC."
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