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"For mechanistic studies of fast organelle dynamics and for multiplex studies of single molecule localization with confocal and super-resolution in living cells, tissues and disease models, the Dragonfly 500 series multi-modal confocal imaging system is the only system fulfilling our application needs. Research necessitates the most sensitive platform available, specifically an integrated system versatile for multiplex single-molecule studies and ultrafast live imaging of mammalian tissue preparations in concert with real-time super-resolution acquisition.\nDragonfly 500 system is unrivalled for ultrafast imaging sensitivity and perfect illumination delivery via the patented Borealis systemTM, offering a non-uniformity of illumination of under 10% over a large (16.6 x 14mm) field of view with all illumination wavelengths (lasers 404nm, 488nm, 561nm, 642, and 730nm) In addition the multi-mode fiber illumination and beam shaping of the Borealis system offer improved illumination throughput and up to 3 times shorter exposure times with equivalent laser powers. Shorter exposure times significantly reduce motion blur artifacts when imaging fast-moving intracellular structures. Borealis is the leading technology in uniform illumination delivery over a sufficiently broad spectral range for high-fidelity multiplex studies.\nThe patented Nikon hardware based Perfect Focus System continuously maintains the focal position on the sample with rapid autofocus, which is critical in fast live cell imaging. Compared to other available autofocus systems, PFS operates on a uniquely long wavelength, which will not interfere with the detection of fluorophore signals up to 800nm. The combination of PFS with the Borealis system offers an unrivaled platform for imaging at longer, near infra-red wavelengths (NIR, 750-800nm emission), with the benefits of deeper tissue penetration in larger samples, much less concern of cell\/tissue autofluorescence, less phototoxicity, more flexibility and\/or higher throughput with greater choice of fluorescent probes, and the possibility of efficient multiplexing of fluorophores essential for our single molecule studies.\nThe ANDOR online implementation of the Super-Resolution Radial Fluctuations (SRRF) technique offers a fast super-resolution option. The Dragonfly is also the only spinning disk system allowing real-time super-resolution imaging at near infra-red wavelengths up to 800nm and at resolutions up to 50nm.\n"
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