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"1. An integrated solution that combines conventional confocal imaging with TCSPC fluorescence lifetime imaging in a single acquisition software application with integrated hardware (i.e. lasers, AOBS, AOTF, and detectors) that support both measurement modalities (intensity & lifetime). ",
"2. For required flexibility in a multi-user core facility, an integrated solution as per point 1 that incorporates a broad range of laser excitation combining a CW 405 nm laser with a freely tuneable pulsed white light laser with a range of 440 nm to 790 nm, and where at least eight laser wavelengths can be used simultaneously.",
"3. For required flexibility in a multi-user core facility and to meet an absolute requirement for simultaneous detection of at least 5 partially spectrally overlapping fluorophores, an integrated solution as per points 1 and 2 which incorporates at least five dedicated high-sensitivity point detectors optimized for an extended detection range from around 400 nm to 850 nm and where for required flexibility the detection range at each detector is freely tuneable. These detectors are required to function in both analogue gain mode and in photon counting mode. ",
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"5. For required flexibility in a multi-user core facility, an integrated solution as per points 1-4 which enables improved imaging capabilities by providing a validated solution for enhanced contrast imaging by fluorescence lifetime-based separation of autofluorescence from specific fluorescence signal, and for enhanced multiplexing imaging capabilities much beyond the simultaneous imaging of 5 partially spectrally overlapping fluorophores by also enabling the differentiation of spectrally overlapping fluorophores from their differences in fluorescence lifetimes. ",
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"Leica STELLARIS 8 with FALCON Fast FLIM, FCS and Resonant Scanner microscope system, supplied by Immuno Diagnostica Oy, is the only instruments in the market that meet all the specific and necessary technical requirements to fulfil the uncompromising needs of the acquiring unit.",
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